Anti-Exonuclease 1 抗体 - N-terminal (ab82314)

製品の概要

  • 製品名Anti-Exonuclease 1 antibody - N-terminal
    Exonuclease 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Exonuclease 1 - N-terminal
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide designed within the N-terminal region of Human Exonuclease 1, conjugated to KLH

  • ポジティブ・コントロール
    • NCI-H460 cell line lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 94 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • 組織特異性Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • 配列類似性Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
  • 発生段階Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
  • 翻訳後修飾Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
  • 細胞内局在Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • exo1 antibody
    • EXO1_HUMAN antibody
    • ExoI antibody
    • Exonuclease 1 antibody
    • Exonuclease I antibody
    • Exonuclease1 antibody
    • HEX1 antibody
    • hExo I antibody
    • hExo1 antibody
    • hExoI antibody
    • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
    see all

Anti-Exonuclease 1 antibody - N-terminal 画像

  • Anti-Exonuclease 1 antibody - N-terminal (ab82314) at 1/50 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

Anti-Exonuclease 1 antibody - N-terminal (ab82314) 使用論文

ab82314 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82314.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"