製品の概要

  • 製品名Anti-Exonuclease 1 antibody
    Exonuclease 1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Exonuclease 1
  • 特異性ab77375 is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-HRNYSPRPESGT

    , corresponding to internal sequence amino acids 372-383 of Human Exonuclease 1 (NP_003677.3; NP_006018.3; NP_569082.1).

  • ポジティブ・コントロール
    • HEK293 and A549 cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab77375 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).
    An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
    • 組織特異性Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
    • 配列類似性Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
    • 発生段階Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
    • 翻訳後修飾Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
    • 細胞内局在Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • exo1 antibody
      • EXO1_HUMAN antibody
      • ExoI antibody
      • Exonuclease 1 antibody
      • Exonuclease I antibody
      • Exonuclease1 antibody
      • HEX1 antibody
      • hExo I antibody
      • hExo1 antibody
      • hExoI antibody
      • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
      see all

    Anti-Exonuclease 1 antibody 画像

    • Anti-Exonuclease 1 antibody (ab77375) at 0.2 µg/ml + A549 cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 94 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Anti-Exonuclease 1 antibody (ab77375) 使用論文

    ab77375 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab77375.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"