製品の概要

  • 製品名Anti-EXOC6B antibody
    EXOC6B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to EXOC6B
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 766-796 of Human EXOC6B (NP_056004.1).

  • ポジティブ・コントロール
    • Mouse heart tissue lysate IF/ICC: Hela cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab116383 was purified through a Protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-EXOC6B antibody 画像

  • ab116383 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116383 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-EXOC6B antibody (ab116383) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 94 kDa

Anti-EXOC6B antibody (ab116383) 使用論文

This product has been referenced in:
  • Wen J  et al. Phenotypic and functional consequences of haploinsufficiency of genes from exocyst and retinoic acid pathway due to a recurrent microdeletion of 2p13.2. Orphanet J Rare Dis 8:100 (2013). WB . Read more (PubMed: 23837398) »

See 1 Publication for this product

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