製品の概要

  • 製品名
    Anti-EXO1 antibody
  • 製品の詳細
    Rabbit polyclonal to EXO1
  • 特異性
    This antibody has been shown to be specific for EXO1.
  • アプリケーション
    適用あり: WB, Indirect ELISAmore details
  • 種交差性
    交差種: Hordeum vulgare
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 395-412 of Barley (Hordeum vulgare) EXO1

  • ポジティブ・コントロール
    • Recombinant EXO1.
  • 特記事項
    For storage at 4°C add an appropriate antibacterial agent.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 68 kDa.
Indirect ELISA 1/10000.

ターゲット情報

  • 関連性
    Family 3 beta-D-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-D-glucosyl residues from non-reducing termini of a range of beta-D-glucans and beta-D-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-D-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme’s surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-D-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.
  • 参照データベース
    • 別名
      • Beta D glucan exohydrolase isoenzyme ExoI antibody

    画像

    • Lane 1 : Anti-EXO1 antibody (ab82533) at 1/5000 dilution
      Lane 2 : Pre-immune sera

      Lane 1 : Recombinant EXO1 (overloaded)
      Lane 2 : Recombinant EXO1 (overloaded)


      Predicted band size : 68 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab82533 has not yet been referenced specifically in any publications.

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