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Carrier coupled exendin 4.
Our Abpromise guarantee covers the use of ab23407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/2000. The antibody binds to exendin 4 when coated on ELISA wells and reacts specifically with exendin 4 in solution giving a Ka of 1.6 x 10E8 in an inhibition ELISA. The binding between ab23407 and exendin 4 is disrupted by 4.5 M MgCl2.|
The concentration of exendin-4 secreted by recombinant L. paracasei was measured by indirect ELISA. Standard exendin-4 (0.2 μg/ml) was used as the coated antigen. Equal volumes (100 μl) of ab23407 (1/4000) and standard exendin-4 (0–100 nmol/l) or culture supernatant samples of L. paracasei L14/pMG76e-exendin-4, preincubated overnight at 4°C, were applied to detect the captured exendin-4. Horseradish peroxidase-conjugated rabbit anti-mouse IgG (100 μl) was used at a 1:10,000 dilution and revealed by reaction with TMBS substrate. Absorption was determined at 450 nm and 630 nm by Multiskan MK3 ELISA plate reader. The exendin-4 concentration was calculated by the standard curve method.