製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to EVI1
  • 特異性
    ab99497 is expected to recognize all reported isoforms
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to internal sequence amino acids 1017-1026 (

    C-DKESLHSTSH

    ) of Human EVI1 isoform 1 (NP_005232.2). The sequence also occurs in NP_001098547.3; NP_004982.2; NP_001157471.1; and NP_001157472.1.

  • ポジティブ・コントロール
    • A549 and HepG2 lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab99497 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 118 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

ターゲット情報

  • 関連性
    EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • AML1 EVI1 antibody
    • Ecotropic viral integration site 1 antibody
    • Ecotropic virus integration 1 site protein antibody
    • Ecotropic virus integration site 1 protein homolog antibody
    • EVI 1 antibody
    • EVI1 antibody
    • MDS1 and EVI1 complex locus antibody
    • MDS1 and EVI1 complex locus protein EVI1 antibody
    • MDS1 and EVI1 complex locus protein MDS1 antibody
    • MDS1 antibody
    • MDS1 EVI1 antibody
    • MGC163392 antibody
    • MGC97004 antibody
    • Myelodysplasia syndrome 1 antibody
    • Myelodysplasia syndrome 1 protein antibody
    • Myelodysplasia syndrome-associated protein 1 antibody
    • Oncogene EVI1 antibody
    • PRDM3 antibody
    • Zinc finger protein Evi1 antibody
    see all

画像

  • Anti-EVI1 antibody (ab99497) at 2 µg/ml (for 1 hour) + A549 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 118 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab99497 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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