製品の概要

  • 製品名Anti-ETS1 antibody [EPR546(2)]
    ETS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR546(2)] to ETS1
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human ETS1.

  • ポジティブ・コントロール
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 50 kDa).
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Transcription factor.
    • 配列類似性Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • 翻訳後修飾Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
      Ubiquitinated; which induces proteasomal degradation.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
      • C ets 1 protein antibody
      • ETS 1 antibody
      • Ets protein antibody
      • ETS1 antibody
      • ETS1 protein antibody
      • ETS1_HUMAN antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLJ10768 antibody
      • P54 antibody
      • Protein C-ets-1 antibody
      • v ets avian erythroblastosis virus E2 oncogene homolog antibody
      • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
      • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
      • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
      see all

    Anti-ETS1 antibody [EPR546(2)] 画像

    • Overlay histogram showing Jurkat cells stained with ab109212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109212, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ETS1 antibody [EPR546(2)] (ab109212) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : PBMC lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa

    Anti-ETS1 antibody [EPR546(2)] (ab109212) 使用論文

    This product has been referenced in:
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"