Store at Room Temperature. The product can be stored for up to 12 months.
Soluble in DMSO to 100 mM, in ethanol to 500 mM, in 1 eq. HCl to 100 mM (with heating) and in water to 1 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - Etomidate (ab120311)
ab7977 staining BAX in RAW 264.7 cells treated with etomidate (ab120311), by ICC/IF. Increase in BAX expression correlates with increased concentration of etomidate, as described in literature. The cells were incubated at 37°C for 24h in media containing different concentrations of ab120311 (etomidate) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7977 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Drexler B et al. Distinct actions of etomidate and propofol at beta3-containing gamma-aminobutyric acid type A receptors. Neuropharmacology57:446-55 (2009).
Read more (PubMed: 19555700) »
van den Burg EH et al. Etomidate reduces initiation of backpropagating dendritic action potentials: implications for sensory processing and synaptic plasticity during anesthesia. J Neurophysiol97:2373-84 (2007).
Read more (PubMed: 17202233) »