製品の概要

  • 製品名
    Anti-ETEA antibody
    ETEA 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to ETEA
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    HTEVLFVQDLTDE

    , corresponding to amino acids 433-445 of Human ETEA.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 機能
    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • 組織特異性
    Broadly expressed, with highest levels in brain.
  • 配列類似性
    Contains 1 UBA domain.
    Contains 1 UBX domain.
  • 細胞内局在
    Cytoplasm. Lipid droplet. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • AI462440 antibody
    • expressed in T cells and eosinophils in atopic dermatitis antibody
    • faf2 antibody
    • FAF2_HUMAN antibody
    • Fas associated factor family member 2 antibody
    • FAS-associated factor 2 antibody
    • KIAA0887 antibody
    • Protein ETEA antibody
    • Protein expressed in T cells and eosinophils in atopic dermatitis antibody
    • Protein expressed in T-cells and eosinophils in atopic dermatitis antibody
    • UBX domain containing 8 antibody
    • UBX domain protein 3B antibody
    • UBX domain-containing protein 3B antibody
    • UBX domain-containing protein 8 antibody
    • UBXD8 antibody
    • UBXN3B antibody
    see all

画像

  • Western blot - Anti-ETEA antibody (ab14759)
    Western blot - Anti-ETEA antibody (ab14759)
    All lanes : Anti-ETEA antibody (ab14759) at 1 µg/ml

    Lane 1 : Human thymus lysate (in RIPA buffer)
    Lane 2 : Mouse thymus lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 51 kDa
    Observed band size: 53 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

    Detected by chemiluminescence.

  • Western blot - Anti-ETEA antibody (ab14759)
    Western blot - Anti-ETEA antibody (ab14759)
    Anti-ETEA antibody (ab14759) at 1 µg/ml + Cell lysates prepared from Jurkat cells at 35 µg

    Predicted band size: 51 kDa



    Western blot showing ab14759 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.

プロトコール

参考文献

ab14759 has not yet been referenced specifically in any publications.

ab14759 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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