製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ETAA1
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    DMPELFPSKT AHVTDQKEIC TFNSKTVKNT SRANTSPDAR LGDSKVLQDL SSKTYDRELI DAEYRFSPNS NKSNK, corresponding to amino acids 398-469 of Human ETAA1 (Q9NY74).

  • ポジティブ・コントロール
    • Human kidney tissue, Human cell line U-2 OS.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: fixation of cells in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

ターゲット情報

  • 組織特異性
    Expressed at high levels in the brain, liver kidney and Ewing tumor cell lines.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Etaa1 antibody
    • ETAA1_HUMAN antibody
    • ETAA16 antibody
    • Ewing''s tumor-associated antigen 1 antibody
    • Ewing''s tumor-associated antigen 16 antibody
    see all

画像

  • ab122245, at a 1/400 dilution, staining ETAA1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab122245, at 4 µg/ml, staining ETAA1 in Human cell line U-2 OS by Immunofluorescence.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

参考文献

ab122245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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