Anti-Estrogen Related Receptor gamma 抗体 - ChIP Grade (ab16366)

製品の概要

  • 製品名Anti-Estrogen Related Receptor gamma antibody - ChIP Grade
    Estrogen Related Receptor gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Estrogen Related Receptor gamma - ChIP Grade
  • 特異性This antibody detects human Estrogen Related Receptor gamma from transfected cells and shows no cross reactivity to ERR1.
  • アプリケーション適用あり: ChIP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    DYEAGQHMEDPRR

    , corresponding to amino acids 401-413 of Human Estrogen Related Receptor gamma. This sequence is completely conserved between human, mouse and rat. (Peptide available as ab39779.)

  • ポジティブ・コントロール
    • COS7 or HEK293 transfected cells. Human brain, kidney or pancreas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human Estrogen Related Receptor gamma peptide (ab39779). Representing Estrogen Related Receptor gamma from COS7 transfected cells.

ターゲット情報

  • 機能Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
  • 組織特異性Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 発生段階Expressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
  • 翻訳後修飾Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity.
    Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781L1617 antibody
    • ERR 3 antibody
    • ERR G2 antibody
    • ERR gamma 2 antibody
    • ERR gamma-2 antibody
    • ERR3 antibody
    • ERR3_HUMAN antibody
    • ERRG 2 antibody
    • ERRG2 antibody
    • ERRgamma antibody
    • ESRRG antibody
    • Estrogen receptor related protein 3 antibody
    • Estrogen receptor-related protein 3 antibody
    • Estrogen-related receptor gamma antibody
    • FLJ16023 antibody
    • KIAA0832 antibody
    • NR3B3 antibody
    • Nuclear receptor subfamily 3 group B member 3 antibody
    see all

Anti-Estrogen Related Receptor gamma antibody - ChIP Grade 画像

  • Lanes 1 - 2 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/500 dilution
    Lane 3 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Mouse kidney cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) 使用論文

ab16366 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. ERR3 (ab16366) is epitope affinity purified, but it appears there are a few non-specific bands around the 54 kDa range, which is why this product is recommended for cells overexpressing the protein. ERR3 has been used ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"