The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1/16000.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Expressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity. Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.