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Our Abpromise guarantee covers the use of ab16363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 52, 37 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human Estrogen Related Receptor alpha peptide (ab39777).|
|ChIP||Use 0.26 µg for µg of chromatin.|
Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg MCF7 whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16363.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Anti-Rabbit HRP (IgG light chain) (ab99697).
Band: 48kDa: Estrogen Related Receptor alpha.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"