Anti-Estrogen Receptor beta 抗体 [EPR3777] (ab92306)


  • 製品名Anti-Estrogen Receptor beta antibody [EPR3777]
    Estrogen Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3777] to Estrogen Receptor beta
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the N terminal of Human Estrogen Receptor beta was used as an immunogen (UniProt ID: Q92731).

  • ポジティブ・コントロール
    • WB: MCF-7 and HeLa cell lysates. IP: HeLa cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Estrogen Receptor beta antibody (HRP) [EPR3777] (ab200599)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab92306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IP 1/10 - 1/120.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
    • 組織特異性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
    • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ER BETA antibody
      • ER-beta antibody
      • Erb antibody
      • ESR B antibody
      • ESR BETA antibody
      • ESR2 antibody
      • ESR2_HUMAN antibody
      • ESRB antibody
      • ESTRB antibody
      • estrogen nuclear receptor beta variant a antibody
      • estrogen nuclear receptor beta variant b antibody
      • estrogen receptor 2 (ER beta) antibody
      • Estrogen receptor 2 antibody
      • estrogen receptor beta 4 antibody
      • Estrogen receptor beta antibody
      • NR3A2 antibody
      • Nuclear receptor subfamily 3 group A member 2 antibody
      see all

    Anti-Estrogen Receptor beta antibody [EPR3777] 画像

    • All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab92306 (purified) at 1/20 immunoprecipitating Estrogen Receptor beta in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 82-95 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/1000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 59 kDa

    Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) 使用論文

    ab92306 has not yet been referenced specifically in any publications.

    Product Wall

    We have not tested ab92306 for its potential cross-reactivity with estrogen receptor alpha. Sequence homology is 66.7% between the immunogen of ER beta and ER alpha, so we would not predict cross-reactivity. However, it is not guaranteed without testin...

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