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Synthetic peptide corresponding to Mouse Estrogen Receptor beta aa 467-485.
Our Abpromise guarantee covers the use of ab5784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).|
|ICC/IF||1/50 - 1/200.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Estrogen Receptor beta (green) with ab5784 at 1/100. Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were incubated with the primary antibody for at least 1 hour at room temperature. A DyLight 488-conjugated goat anti-rabbit IgG (1/200) secondary antibody was used. Nuclei (blue) were stained with Hoechst. 20X magnification.
Shows a Western blot of Estrogen Receptor beta on HEK cell extract using ab5784.
ab5784 has not yet been referenced specifically in any publications.