Anti-Estrogen Receptor beta 抗体 [14C8] (ab288)

製品の概要

  • 製品名
    Anti-Estrogen Receptor beta antibody [14C8]
    Estrogen Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14C8] to Estrogen Receptor beta
  • 特異性
    14C8 is specific for human ER-beta. The antibody has been tested in WB against recombinant protein. It has not yet been tested in our laboratory using any cell lysates and several publications suggest it can give weak bands in breast tumour extracts lysate. Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
  • アプリケーション
    適用あり: WB, Flow Cyt, IHC-P, ICC/IF, ChIP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human, Monkey, Baboon
  • 免疫原

    Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-beta expressed in E. coli.

  • ポジティブ・コントロール
    • WB: Recombinant fusion protein. IHC-P: endocervical epithelium, Lobular breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
Flow Cyt 1/25.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 組織特異性
    Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody [14C8] 画像

  • ab288 staining endocervical epithelium by IHC-P.
  • All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution

    Lane 1 : Non-transfected 293T whole cell extracts
    Lane 2 : Transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.


    Performed under non-reducing conditions.

    Predicted band size : 59 kDa
  • ab288 staining infiltrating lobular carcinoma of the breast by IHC-P.

Anti-Estrogen Receptor beta antibody [14C8] (ab288) 使用論文

This product has been referenced in:
  • Nelson AW  et al. Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity. Mol Cell Endocrinol 440:138-150 (2017). WB, IP ; Human . Read more (PubMed: 27889472) »
  • Schüler-Toprak S  et al. Effect of estrogen receptor ß agonists on proliferation and gene expression of ovarian cancer cells. BMC Cancer 17:319 (2017). WB ; Human . Read more (PubMed: 28482871) »

See all 53 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (OV90)
Permeabilization
Yes - 0.25% Triton-X100 in PBS
Specification
OV90
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0
Sample
Baboon Tissue sections (fetal ovary)
Specification
fetal ovary
Permeabilization
No
Fixative
Formaldehyde
Username

Ms. Marcia Burch

Verified customer

投稿 Sep 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast cancer)
Specification
Breast cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization
Yes - Wash Buffer with tween
Username

Mr. Rudolf Jung

Verified customer

投稿 Dec 03 2012

Thank you for your enquiry.

I can confirm that we recommend not to dilute antibodies before storage unless is states to do so on the individual datasheet. For all antibodies, we suggest to always follow instructions provided on the individua...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


Please apologize for the delay of my answer: Anja is on her well deserved holiday now, so I ...

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Thank you for your enquiry and your interest in our products.
The effect of formalin fixation and the long-term storage on the detectability of different proteins have been studies for some time. The longer the tissues are fixed and stored in form...

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Thank you for taking time to liaise with this customer to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. Having reviewed this case, I would like to offer some suggestions to help optimize the re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (vaginal)
Specification
vaginal
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton in PBS
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 17 2011

Thank you for your inquiry. The 6x his tag was not removed before the antibody was raised. If I can help provide any further information please let me know.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer)
Specification
breast cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: • Microwave slides in 10mM citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. W Xu

Verified customer

投稿 May 11 2010

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