Anti-Estrogen Receptor alpha 抗体 [SP1], prediluted (ab27595)


  • 製品名
    Anti-Estrogen Receptor alpha antibody [SP1], prediluted
    Estrogen Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP1] to Estrogen Receptor alpha, prediluted
  • 由来種
  • 特異性
    ab27595 recognises estrogen receptor alpha (ER).
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor alpha. Synthetic peptide derived from C-terminal of human estrogen receptor alpha.

  • ポジティブ・コントロール
    • Breast carcinomas



Our Abpromise guarantee covers the use of ab27595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

USE NEAT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.


  • 機能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 細胞内局在
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all


  • Immunohistochemical staining of human breast carcinoma labelling Estrogen Receptor alpha [SP1] with ab93739 at 1:200 dilution.

  • Staining of human breast carcinoma using ab27595
  • Anti-Estrogen Receptor alpha antibody [SP1], prediluted (ab27595) at 1/25 dilution + lysate prepared from MCF7 cells

    Image produced using the diluted version of this antibody, ab16660.


This product has been referenced in:
  • Gu J  et al. Knockdown of HIF-1a by siRNA-expressing plasmid delivered by attenuated Salmonella enhances the antitumor effects of cisplatin on prostate cancer. Sci Rep 7:7546 (2017). Read more (PubMed: 28790395) »
  • Tian X  et al. Identification of a hybrid myocardial zone in the mammalian heart after birth. Nat Commun 8:87 (2017). Read more (PubMed: 28729659) »

See all 12 Publications for this product

レビューと Q&A

ab27595 is specific for ER-alpha. We do not have any experimental data regarding the cross-reactivity of ab27595 with ER-beta. But there is no overlap between the immunogen for ab27595 and the ER-beta protein (Q92731), so theoretically it should...

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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Thank you for contacting us.

As antigen retrieval solution, citrate buffer pH 6.0 was used.
Please see below for the detailed protocol:

Pretreatment (Antigen retrieval):

Step 1. Heat slides in citrate buffer in micr...

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Thank you for your inquiry. No, based on immunogen sequence ab27595 will not recognize a sequence between aa 302-553. I am sorry that this information could not be more helpful. Please contact me with any other questions.