リコンビナント
RabMAb

Anti-Estrogen Receptor alpha 抗体 [EPR4097] - ChIP Grade (ab108398)

製品の概要

  • 製品名
    Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade
    Estrogen Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4097] to Estrogen Receptor alpha - ChIP Grade
  • 特異性
    For Mouse, ab108398 is recommended for WB only. Rat cross reactivity tested by WB only.
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ChIP, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Estrogen Receptor alpha aa 1-300. Recombinant Human Estrogen Receptor alpha protein.

  • ポジティブ・コントロール
    • WB: MCF7 and T47-D cell lysates. IHC-P: Human breast ductal infiltrating carcinoma and normal breast tissues. ICC/IF: MCF-7 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-Estrogen Receptor alpha antibody (Alexa Fluor® 488) [EPR4097] (ab205850)

    Anti-Estrogen Receptor alpha antibody (Alexa Fluor® 647) [EPR4097] (ab205851)

    Anti-Estrogen Receptor alpha antibody (Alexa Fluor® 594) [EPR4097] (ab207046)

    Anti-Estrogen Receptor alpha antibody (Alexa Fluor® 568) [EPR4097] (ab207261)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.

For unpurified use at 1/200 - 1/1000.

IHC-P 1/250.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/30.

Flow Cyt Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF 1/250.

For unpurified use at 1/30.

ターゲット情報

  • 機能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 細胞内局在
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

画像

  • All lanes : purified at 1/2000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : T47-D cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 66 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling Estrogen Receptor alpha with purified ab108398 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling Estrogen Receptor alpha (green) with purified ab108398 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • ab108398 staining Estrogen Receptor alpha in the human cell line MCF-7(human breast carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ChIP analysis using unpurified ab108398 binding Estrogen Receptor alpha in MCF7 cells derived from Human breast carcinoma. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: Estrogen treated MCF7 cells tested at PS2 promoter.
    Negative Control:IgG ChIP and ethanol-depleted cells tested at PS2 promoter.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast ductal infiltrating carcinoma tissue labelling Estrogen Receptor alpha with unpurified ab108398.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human normal tonsil tissue. Unpurified ab108398 shows negative staining.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human colonic adenocarcinoma tissue. Unpurified ab108398 shows negative staining.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human lung adenocarcinoma tissue. Unpurified ab108398 shows negative staining.

  • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human cervical carcinoma tissue. Unpurified ab108398 shows negative staining.

参考文献

This product has been referenced in:
  • Castle J  et al. Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression. PLoS One 12:e0175647 (2017). IHC ; Human . Read more (PubMed: 28422972) »
  • Ullah K  et al. Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium. J Mol Endocrinol 59:105-119 (2017). IF . Read more (PubMed: 28539318) »

See all 2 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (Pig Mammary Epithelial Cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
15 µg
Specification
Pig Mammary Epithelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Spodoptera frugiperda (SF9 cells) Cell lysate - whole cell (sf9 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100000 cells
Treatment
30 nM Bortezomib for 16 hours
Specification
sf9 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat intestinal epithelial cells (RIE-1))
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100000 cells
Treatment
100 nM Bortezomib for 16 hours
Specification
rat intestinal epithelial cells (RIE-1)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary cell)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100000 cells
Treatment
100 nM Bortezomib for 16 hours
Specification
Chinese hamster ovary cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2016

Application
IHC - Wholemount
Sample
Human Tissue (Invasive ductal carcinoma)
Specification
Invasive ductal carcinoma
Username

Abcam user community

Verified customer

投稿 Dec 19 2015

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS-7 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100000 cells
Treatment
30 nM Bortezomib for 24 hours
Specification
COS-7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (mammary cell line (MCF7))
Specification
mammary cell line (MCF7)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
Estrogen treated MCF7 cells tested at PS2 promoter
Negative control
IgG ChIP and ethanol-depleted cells tested at PS2 promoter
Username

Abcam user community

Verified customer

投稿 Mar 14 2013

Thank you for your inquiry. This antibody is tested and guaranteed for ICC. We did not test this antibody for IF but it should be suitable if the correct secondary antibody (anti rabbit that is tested for IF) is used. I hope thi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録