Anti-Estrogen Receptor alpha 抗体 (ab3575)
製品の概要
-
製品名Anti-Estrogen Receptor alpha antibody
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Estrogen Receptor alpha
-
アプリケーション適用あり: ICC/IF, WB, Flow Cyt, ICC, IHC-Pmore details
-
種交差性交差種: Mouse, Rat, Human, Pig, Deer
交差が予測される動物種: Hamster, Cow, Cat
-
免疫原
-
ポジティブ･コントロール
- ICC: Human coronary artery smooth muscle cells (See Han et al). IHC-P: Rat prostate tissue. ICC/IF: T-47D cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast ductal carcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Conjugation kits
-
Immunizing Peptide (Blocking)
-
Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab3575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 0.5 mg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23768049 | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
-
機能Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
-
配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
-
翻訳後修飾Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
- Information by UniProt
-
参照データベース
- Entrez Gene: 407238 Cow
- Entrez Gene: 101825539 Hamster
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 397435 Pig
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P49884 Cow
see all -
別名
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
see all
Anti-Estrogen Receptor alpha antibody 画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (ab3575)ab3575 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3575 at 5 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)IHC image of Estrogen Receptor alpha staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab3575, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
-
Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique
Predicted band size : 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
-
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (ab3575)4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
Anti-Estrogen Receptor alpha antibody (ab3575) 使用論文
This product has been referenced in:
- Ghebeh H et al. Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers. BMC Cancer 13:289 (2013). Flow Cyt ; Human . Read more (PubMed: 23768049) »
- Anagnostis A & Papadopoulos AI Effects of a diet rich in sesame ( Sesamum indicum) pericarp on the expression of oestrogen receptor alpha and oestrogen receptor beta in rat prostate and uterus. Br J Nutr 102:703-8 (2009). WB ; Rat . Read more (PubMed: 19309532) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"