製品の概要

  • 製品名
    Anti-Estrogen Receptor alpha antibody
    Estrogen Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Estrogen Receptor alpha
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WB, Flow Cyt, ICC, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig, Deer
    交差が予測される動物種: Hamster, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 21-32.
    Sequence:

    NELEPLNRPQLK


    Database link: P03372
    (Peptide available as ab5847)

  • ポジティブ・コントロール
    • ICC: Human coronary artery smooth muscle cells (See Han et al). IHC-P: Rat prostate tissue. ICC/IF: T-47D cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast ductal carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 0.5 mg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).
Flow Cyt Use at an assay dependent concentration. PubMed: 23768049

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 細胞内局在
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

画像

  • IHC image of Estrogen Receptor alpha staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab3575, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.

  • All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution

    Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
    Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
    Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
    Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    Blocked with 5% milk in TBST for at least 1 hour at room temperature.

  • 4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.

参考文献

This product has been referenced in:
  • Tang YQ  et al. Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats. BMC Complement Altern Med 18:118 (2018). Read more (PubMed: 29609586) »
  • Ghebeh H  et al. Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers. BMC Cancer 13:289 (2013). Flow Cyt ; Human . Read more (PubMed: 23768049) »

See all 6 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Cancer Metastasis in Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph6 citrate
Permeabilization
No
Specification
Breast Cancer Metastasis in Lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Victoria Bridgeman

Verified customer

投稿 May 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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