製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ESE1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: SGWKEEEVLQSRN, corresponding to C terminal amino acids 359-371 of Human ESE1.

  • ポジティブ・コントロール
    • NCI-H460 lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human ESE1 peptide (ab22940).
This antibody can be used with studies using peptide ab2934.
ELISA 1/32000.

ターゲット情報

  • 機能
    Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
  • 組織特異性
    Expressed exclusively in tissues containing a high content of terminally differentiated epithelial cells including mammary gland, colon, trachea, kidney, prostate, uterus, stomach and skin.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • ドメイン
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 細胞内局在
    Cytoplasm. Nucleus. Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E74 like factor 3 antibody
    • E74 like factor 3 ets domain transcription factor antibody
    • E74 like factor 3 ets domain transcription factor epithelial specific antibody
    • E74 like factor 3 ETS domain transcription factor serine box epithelial specific antibody
    • E74-like factor 3 antibody
    • Elf3 antibody
    • ELF3_HUMAN antibody
    • Epithelial restricted with serine box antibody
    • Epithelial-restricted with serine box antibody
    • Epithelium restricted Ets protein ESX antibody
    • Epithelium specific Ets factor 1 antibody
    • Epithelium specific Ets transcription factor 1 antibody
    • Epithelium-restricted Ets protein ESX antibody
    • Epithelium-specific Ets transcription factor 1 antibody
    • EPR 1 antibody
    • EPR1 antibody
    • ERT antibody
    • ESE-1 antibody
    • ESX antibody
    • Ets domain transcription factor serine box antibody
    • Ets domain transcription factor serine box epithelial specific antibody
    • Ets transcription factor antibody
    • ETS-related transcription factor Elf-3 antibody
    • jen antibody
    • MGC139318 antibody
    see all

画像



  • Predicted band size : 42 kDa


    ab2934 staining (3µg/ml) of NCI-H460 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

プロトコール

参考文献

ab2934 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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