The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). Use under reducing conditions.
The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
6 kDa early secretory antigenic target antibody
6 kDa early secretory antigenic target ESXA antibody
Tan K et al. Comparison of BCG prime-DNA booster and rBCG regimens for protection against tuberculosis. Hum Vaccin Immunother10:391-8 (2014).
Read more (PubMed: 24192709) »
Nguyen YH et al. Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay. Sci Rep2:635 (2012).
Read more (PubMed: 22957139) »