製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ESAT6
  • 特異性
    This antibody reacts with recombinant ESAT6 produced in E.coli.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    Reacts with Mycobacterium tuberculosis (strain H37Rv) and Mycobacterium bovis. Not yet tested in other species.
  • 免疫原

    Full length protein (Rv3875) from Mycobacterium tubercolosis.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). Use under reducing conditions.

ターゲット情報

  • 関連性
    The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • 細胞内局在
    Secreted
  • 別名
    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Tan K  et al. Comparison of BCG prime-DNA booster and rBCG regimens for protection against tuberculosis. Hum Vaccin Immunother 10:391-8 (2014). Read more (PubMed: 24192709) »
  • Nguyen YH  et al. Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay. Sci Rep 2:635 (2012). Functional Studies . Read more (PubMed: 22957139) »

See all 2 Publications for this product

レビューと Q&A

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Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Specification
Cell lysate, supernatant
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Feb 18 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
Total protein in input
1000 µg
Specification
M. tuberculosis culture filtrate
Immuno-precipitation step
Protein G
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投稿 Jul 11 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
15 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

投稿 Jul 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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