製品の概要

  • 製品名
    Anti-ESAT6 antibody [11G4]
    ESAT6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [11G4] to ESAT6
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    Reacts with Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium szulgai and Mycobacterium marinum.
  • 免疫原

    Tissue/ cell preparation: PPD from Mycobacterium tuberculosis (Tuberculin PPD).

  • エピトープ
    This antibody binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 of ESAT6.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)
    Protein A/G purified.
  • ポリ/モノ
    モノクローナル
  • クローン名
    11G4
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab26246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa.

Detects a band of approximately 10 kDa in short term culture of M. tuberculosis.

ELISA 1/3200.

ターゲット情報

  • 関連性
    The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • 細胞内局在
    Secreted
  • 別名
    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

画像

  • All lanes : Anti-ESAT6 antibody [11G4] (ab26246) at 1/5000 dilution

    Lane 1 : M. tuberculosis culture filtrate
    Lane 2 : M. tuberculosis culture filtrate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse monoclonal at 1/100000 dilution
    Developed using the ECL technique

    Observed band size : 11 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

プロトコール

参考文献

This product has been referenced in:
  • Aguilo N  et al. Reactogenicity to major tuberculosis antigens absent in BCG is linked to improved protection against Mycobacterium tuberculosis. Nat Commun 8:16085 (2017). Read more (PubMed: 28706226) »
  • Ramsdell TL  et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol 427:1119-32 (2015). Read more (PubMed: 24979678) »

See all 13 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mycobacterium tuberculosis Cell lysate - other (M. tuerculosis culture filtrate)
Total protein in input
1000 µg
Specification
M. tuerculosis culture filtrate
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 Jul 11 2012

Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
10 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 27 2012

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

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Thank you for contacting us. The optimal dilution will depend on the nature and amount of your sample but I recommend starting with a dilution of 1/500 (2 ug/ml). Please do not hesitate to contact us if you need any more advice or inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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