製品の概要

  • 製品名Anti-ESAT6 antibody [11G4]
    ESAT6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [11G4] to ESAT6
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    Reacts with Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium szulgai and Mycobacterium marinum.
  • 免疫原

    Tissue/ cell preparation: PPD from Mycobacterium tuberculosis (Tuberculin PPD).

  • エピトープThis antibody binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 of ESAT6.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)Protein A/G purified.
  • ポリ/モノモノクローナル
  • クローン名11G4
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab26246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa.

Detects a band of approximately 10 kDa in short term culture of M. tuberculosis.

ELISA 1/3200.

ターゲット情報

  • 関連性The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
  • 細胞内局在Secreted
  • 別名
    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

Anti-ESAT6 antibody [11G4] 画像

  • All lanes : Anti-ESAT6 antibody [11G4] (ab26246) at 1/5000 dilution

    Lane 1 : M. tuberculosis culture filtrate
    Lane 2 : M. tuberculosis culture filtrate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse monoclonal at 1/100000 dilution
    Developed using the ECL technique

    Observed band size : 11 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-ESAT6 antibody [11G4] (ab26246) 使用論文

This product has been referenced in:
  • Kennedy GM  et al. A novel ESX-1 locus reveals that surface-associated ESX-1 substrates mediate virulence in Mycobacterium marinum. J Bacteriol 196:1877-88 (2014). Read more (PubMed: 24610712) »
  • Solans L  et al. The PhoP-dependent ncRNA Mcr7 modulates the TAT secretion system in Mycobacterium tuberculosis. PLoS Pathog 10:e1004183 (2014). WB . Read more (PubMed: 24874799) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mycobacterium tuberculosis Cell lysate - other (M. tuerculosis culture filtrate)
Total protein in input 1000 µg
Specification M. tuerculosis culture filtrate
Immuno-precipitation step Protein G
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Verified customer

投稿 Jul 11 2012

Application Western blot
Sample Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount 10 µg
Specification M.tuberculosis culture filtrate
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Jun 27 2012

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

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Thank you for contacting us. The optimal dilution will depend on the nature and amount of your sample but I recommend starting with a dilution of 1/500 (2 ug/ml). Please do not hesitate to contact us if you need any more advice or inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"