製品の概要

  • 製品名Anti-ERp57 antibody [MaP.Erp57]
    ERp57 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MaP.Erp57] to ERp57
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, IP, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • HeLa Heat Shocked Cell Lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Hela.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is affinity purified.
  • ポリ/モノモノクローナル
  • クローン名MaP.Erp57
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 61 kDa. This concentration is sufficient for the detection of ERp57 in 20µg of HeLa heat shocked cell lysate by colorimetric analysis.
IP Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 23401559
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-ERp57 antibody [MaP.Erp57] 画像

  • ICC/IF image of ab13506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab13506 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 61 kDa
    Observed band size : 61 kDa

    This image is courtesy of an Abreview submitted by Pawel Mazur

    ERp57 antibody (ab13506) at 1/1000 dilution (in PBST) for 8 hours at 4°C + lysate of mouse of pancreatic cancer cell line at 25 µg.

    Secondary
    An HRP conjugated Sheep anti-mouse IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under non-reducing conditions.

    Exposure time: 1 minute

    Blocking Step: 10% Milk for 1 hour at room temperature.

    See Abreview

  • ab13506 staining ERp57 in Mouse duodenum, pancreas, and pancreatic neoplastic tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone:methanol (1:1), permeabilized with PBS + Triton X-100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab13506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13506, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-ERp57 antibody [MaP.Erp57] (ab13506) 使用論文

This product has been referenced in:
  • Boyle LH  et al. Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway. Proc Natl Acad Sci U S A 110:3465-70 (2013). ICC/IF ; Mouse . Read more (PubMed: 23401559) »
  • Pehar M  et al. Proteomic assessment shows that many endoplasmic reticulum (ER)-resident proteins are targeted by N(epsilon)-lysine acetylation in the lumen of the organelle and predicts broad biological impact. J Biol Chem 287:22436-40 (2012). WB, IP . Read more (PubMed: 22628546) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
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投稿 Jun 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse duodenum, pancreas, and pancreatic neoplasti)
Specification Mouse duodenum, pancreas, and pancreatic neoplasti
Fixative acetone+methanol 1:1
Permeabilization Yes - PBS + Triton X-100 0.025%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 Jul 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human pancreatic cancer cell lines)
Loading amount 30 µg
Specification human pancreatic cancer cell lines
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jul 05 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas, liver, pancreatic tumor)
Specification pancreas, liver, pancreatic tumor
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 Jun 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (pancreatic cancer)
Loading amount 25 µg
Specification pancreatic cancer
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 Jun 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (B-LCL)
Loading amount 80000 cells
Specification B-LCL
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Jun 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"