製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERO1L
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide from the N-terminal region of human ERO1L

  • ポジティブ・コントロール
    • 293T, INS-1, and MIN6 cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 1% BSA, 99% Borate buffered saline
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 機能
    Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
  • 組織特異性
    Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
  • 配列類似性
    Belongs to the EROs family.
  • 翻訳後修飾
    N-glycosylated.
    The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
  • 細胞内局在
    Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Endoplasmic oxidoreductin 1 like protein antibody
    • Endoplasmic oxidoreductin-1-like protein antibody
    • Endoplasmic reticulum oxidoreductase 1 alpha antibody
    • Endoplasmic reticulum oxidoreductin 1-like antibody
    • ERO1 alpha antibody
    • ERO1 L antibody
    • ERO1 Lalpha antibody
    • ERO1 like protein alpha antibody
    • ERO1-alpha antibody
    • ERO1-L antibody
    • ERO1-L-alpha antibody
    • ERO1-like (S. cerevisiae) antibody
    • ERO1-like alpha antibody
    • ERO1-like protein alpha antibody
    • ERO1-like, S. cerevisiae, homolog of, alpha antibody
    • ERO1A antibody
    • ERO1A_HUMAN antibody
    • ERO1L antibody
    • ERO1LA antibody
    • Oxidoreductin 1 Lalpha antibody
    • Oxidoreductin-1-L-alpha antibody
    • PRO865 antibody
    • UNQ434 antibody
    see all

画像

  • Anti-ERO1L antibody (ab81959) at 1/1000 dilution + 293T cell lysate

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

プロトコール

参考文献

This product has been referenced in:
  • Angelucci S  et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci 8:18 (2010). WB ; Human . Read more (PubMed: 20346146) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Loading amount
30 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Dec 18 2012

Thank you for providing that information.

As I mentioned previously, with the exception of ab79483, we do guarantee that these antibodies should work in western blot on rat tissue. If we cannot resolve the issue you are having with the antibod...

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Thank you for contacting Abcam.

I am sorry that you are having some issues with our antibodies. All of the antibodies you mentioned should work in western blot in various species, however only ab30315 is known to work in IHC-P. Below is a list...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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