製品の概要

  • 製品名Anti-ERO1L antibody
    ERO1L 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ERO1L
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: DISQCGRRDC AVKPCQSDEV PDGIKSASYK YSEEANNLIE ECEQAERLGA VDESLSEETQ KAVLQWTKHD DSSDNFCEAD DIQSPEAEY, corresponding to amino acids 90-179 of Human ERO1L

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
    • 組織特異性Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
    • 配列類似性Belongs to the EROs family.
    • 翻訳後修飾N-glycosylated.
      The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
    • 細胞内局在Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Endoplasmic oxidoreductin 1 like protein antibody
      • Endoplasmic oxidoreductin-1-like protein antibody
      • Endoplasmic reticulum oxidoreductin 1-like antibody
      • ERO1 alpha antibody
      • ERO1 L antibody
      • ERO1 Lalpha antibody
      • ERO1 like protein alpha antibody
      • ERO1-alpha antibody
      • ERO1-L antibody
      • ERO1-L-alpha antibody
      • ERO1-like (S. cerevisiae) antibody
      • ERO1-like alpha antibody
      • ERO1-like protein alpha antibody
      • ERO1-like, S. cerevisiae, homolog of, alpha antibody
      • ERO1A antibody
      • ERO1A_HUMAN antibody
      • ERO1L antibody
      • ERO1LA antibody
      • Oxidoreductin 1 Lalpha antibody
      • Oxidoreductin-1-L-alpha antibody
      • PRO865 antibody
      • UNQ434 antibody
      see all

    Anti-ERO1L antibody 画像



    • Predicted band size : 54 kDa
      ERO1L antibody (ab57177) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-ERO1L antibody (ab57177) 使用論文

    This product has been referenced in:
    • Zhang L  et al. Different interaction modes for protein-disulfide isomerase (PDI) as an efficient regulator and a specific substrate of endoplasmic reticulum oxidoreductin-1a (Ero1a). J Biol Chem 289:31188-99 (2014). Read more (PubMed: 25258311) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"