Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-ERM / Etv5 antibody 画像
Western blot - ERM / Etv5 antibody (ab50393)
Lane 1 :
MarkerLane 2 :
Anti-ERM / Etv5 antibody (ab50393) at 1 µg/mlLane 1 :
As aboveLane 2 :
293T cell lysate at 10 µgSecondaryLane 1 :
HRP conjugated anti-Rabbit IgG at 1/50000-1/100000 dilutionLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50000-1/100000 dilution Predicted band size :
58 kDaObserved band size :
50 kDa (why is the actual band size different from the predicted?
Anti-ERM / Etv5 antibody (ab50393) 使用論文
has not yet been referenced specifically in any publications.
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