製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERM / Etv5
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 244-404 of Human ERM/ Etv5 (AAH07333)

  • ポジティブ・コントロール
    • A431, Jurkat, Raji cell lysate, PC14 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 22933516
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.

ターゲット情報

Anti-ERM / Etv5 antibody 画像

  • Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ab102010 at 1/100 dilution.
  • Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 58 kDa

Anti-ERM / Etv5 antibody (ab102010) 使用論文

This product has been referenced in:
  • Diaferia GR  et al. Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer. EMBO J 35:595-617 (2016). IHC-P ; Human . Read more (PubMed: 26769127) »
  • Fanslow DA  et al. Genome editing in mouse spermatogonial stem/progenitor cells using engineered nucleases. PLoS One 9:e112652 (2014). ICC/IF . Read more (PubMed: 25409432) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (pancreatic tumor cells)
Specification
pancreatic tumor cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
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投稿 Aug 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic tumor cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
50 µg
Specification
pancreatic tumor cells
Blocking step
odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Aug 01 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Prepubertal testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Prepubertal testis
Blocking step
5% serum + 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Mar 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Pancreatic Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Mouse Pancreatic Tumor
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Formaldehyde
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投稿 Jan 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatogonia)
Specification
Spermatogonia
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton/PBS
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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投稿 Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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