製品の概要

  • 製品名
    Anti-ERK5 (phospho T218 + Y220) antibody
    ERK5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ERK5 (phospho T218 + Y220)
  • 特異性
    Some cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types.
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from the region of human ERK5 that contains threonine 218 and tyrosine 220.

  • ポジティブ・コントロール
    • HEK293 cells transiently co-transfected with plasmids expressing ERK5 kinase domain (ERK5kin)and constitutively activated MEK5 (MEK5D-D).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 88 kDa. Due to the low abundance and low levels of activation of endogenous ERK5, over-expression or immunoprecipitation may be required.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
  • 組織特異性
    Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメイン
    The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾
    Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates to the nucleus upon activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Big MAP kinase 1 antibody
    • BMK 1 antibody
    • BMK 1 kinase antibody
    • BMK-1 antibody
    • BMK1 antibody
    • BMK1 Kinase antibody
    • EC 2.7.11.24 antibody
    • ERK 4 antibody
    • ERK 5 antibody
    • ERK-5 antibody
    • ERK4 antibody
    • ERK5 antibody
    • Extracellular signal regulated kinase 5 antibody
    • Extracellular signal-regulated kinase 5 antibody
    • MAP kinase 7 antibody
    • MAPK 7 antibody
    • MAPK7 antibody
    • Mitogen activated protein kinase 7 antibody
    • Mitogen-activated protein kinase 7 antibody
    • MK07_HUMAN antibody
    • OTTHUMP00000065906 antibody
    • OTTHUMP00000065907 antibody
    • PRKM 7 antibody
    • PRKM7 antibody
    • PROTEIN KINASE, MITOGEN-ACTIVATED, 7 antibody
    see all

Anti-ERK5 (phospho T218 + Y220) antibody 画像



  • Predicted band size : 88 kDa


    Peptide Competition: Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA TBST buffer overnight at 4oC, then were incubated with the ab5686 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), a generic phosphotyrosine-containing peptide (4), the phosphopeptide derived from the corresponding region of ERK1&2 (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStarTM detection method. The data show that while there is some cross-reactivity with ERK1&2, only the phosphopeptide corresponding to ERK5 [pTpY218/220] completely blocks the antibody signal, demonstrating the specificity of the antibody. NOTE: The antibody signal appears at ~50 kDa as this is the molecular weight of the transiently transfected ERK5 kinase domain.

Anti-ERK5 (phospho T218 + Y220) antibody (ab5686) 使用論文

This product has been referenced in:
  • Govarts C  et al. Analysis of antibody reactivity in paired cerebrospinal fluid and serum of a relapsing remitting multiple sclerosis patient. Autoimmunity 42:699-704 (2009). IHC-Fr ; Human . Read more (PubMed: 19835487) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% PBS-Triton X100
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ricardo Santamaría

Verified customer

投稿 Apr 11 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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