製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERK2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Mouse)

  • ポジティブ・コントロール
    • A431 cells, Breast carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
IHC-P
  • 追加情報
    IHC-P: 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM
    citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • 配列類似性
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • ドメイン
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻訳後修飾
      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ERK 2 antibody
      • ERK antibody
      • ERK-2 antibody
      • ERT1 antibody
      • Extracellular Signal Regulated Kinase 2 antibody
      • Extracellular signal-regulated kinase 2 antibody
      • MAP kinase 1 antibody
      • MAP kinase 2 antibody
      • MAP kinase isoform p42 antibody
      • MAPK 1 antibody
      • MAPK 2 antibody
      • Mapk1 antibody
      • MAPK2 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 2 antibody
      • MK01_HUMAN antibody
      • P38 antibody
      • P40 antibody
      • P41 antibody
      • p42-MAPK antibody
      • P42MAPK antibody
      • PRKM1 antibody
      • PRKM2 antibody
      • protein kinase, mitogen-activated, 1 antibody
      • protein kinase, mitogen-activated, 2 antibody
      • protein tyrosine kinase ERK2 antibody
      see all

    画像

    • ab15282, at 1/200 dilution, staining ERK2 in breast carcinoma by IHC-P.

    参考文献

    This product has been referenced in:
    • Fu Y  et al. Functional ectopic neuritogenesis by retinal rod bipolar cells is regulated by miR-125b-5p during retinal remodeling in RCS rats. Sci Rep 7:1011 (2017). Read more (PubMed: 28432360) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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