製品の概要

  • 製品名Anti-ERK2 antibody [4C11]
    ERK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C11] to ERK2
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
  • 免疫原

    Purified recombinant fragment of human ERK2 expressed in E. Coli.

  • ポジティブ・コントロール
    • HeLa cell lysate. Human lung and breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000.
IHC-Fr 1/500 - 1/2000.
WB 1/500 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Anti-ERK2 antibody [4C11] 画像

  • All lanes : Anti-ERK2 antibody [4C11] (ab119358)

    Lane 1 : HeLa cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : A549 cell lysate
    Lane 7 : NTERA-2 cell lysate
    Lane 8 : SMMC-7721 cell lysate


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab119358 staining ERK2 in Human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic staining with DAB staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab119358 staining ERK2 in Eca-109 cells by Immunocytochemistry/ Immunofluorescense.

Anti-ERK2 antibody [4C11] (ab119358) 使用論文

ab119358 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"