Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 [E337] (ab32538)


  • 製品名Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337]
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • 特異性The antibody detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204 and ERK2 phosphorylated on Threonine 185 and Tyrosine 187.
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187).

  • ポジティブ・コントロール
    • WB: Serum starved A431 cell lysate treated with EGF. IHC-P: Human thyroid gland cancer tissue. ICC/IF: A431 cells +- EGF.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab32538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42 , 44 kDa).Can be blocked with Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187) peptide (ab205613).
IHC-P 1/50.
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/250.
  • 追加情報Is unsuitable for IP.
  • Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] 画像

    • Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/500 dilution
      Lane 2 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/2000 dilution
      Lane 3 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/10000 dilution

      Lane 1 : A431 treated with EGF for 10 minutes
      Lane 2 : A431 treated with EGF for 10 minutes
      Lane 3 : A431 treated with EGF for 10 minutes

      Lysates/proteins at 0.1 µg/ml per lane.

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 42 , 44 kDa
      Observed band size : 42.44 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

      First-antibody diluted with 1% BSA.


    • developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 , 44 kDa

      Exposure time : 16 minutes

      THP1 cells were incubated at 37°C for 3 minutes with vehicle control (0 μM) and different concentrations of ZK 756326 (ab120814). Increased expression of ERK1 (phospho T202 + Y204 ) + ERK2 (phospho T185 + Y187) (ab32538) in THP1 cells correlates with an increase in ZK 756326 concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32538 at 1/500 dilution and ab17942 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) (ab32538) at dilution of 1:50.

    • Immunocytochemistry/Immunofluorescence analysis of 4% paraformaldehyde A431+-EGF(100ng/ml,5min) labelling Erk1 (pT202/pY204) + Erk2 (pT185/pY187) with ab32538 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 Goat-Anti-Rabbit IgG (ab150077) at dilution of 1/400. The counter stain was done with DAPI (blue).

    • Overlay histogram showing HeLa cells stained with ab32538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32538, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) 使用論文

    This product has been referenced in:
    • Wang ZH  et al. Sunitinib mesylate inhibits proliferation of human colonic stromal fibroblasts in vitro and in vivo. J Zhejiang Univ Sci B 15:701-12 (2014). WB ; Human . Read more (PubMed: 25091988) »
    • Assi J  et al. Transglutaminase 2 overexpression in tumor stroma identifies invasive ductal carcinomas of breast at high risk of recurrence. PLoS One 8:e74437 (2013). IHC-P ; Human . Read more (PubMed: 24058567) »

    See all 4 Publications for this product

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