Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 (ab16869)

製品の概要

  • 製品名
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • 特異性
    This antibody recognises ERK1 phosphorylated at Thr202 and Tyr204 and ERK2 phosphorylated at Thr185 and Thr187.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • NIH3T3 cell lysates stimulated with PDGF.

アプリケーション

Our Abpromise guarantee covers the use of ab16869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 43 kDa.

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  • Western Blot analysis of NIH3T3 cell lysates (10µg) stimulated with PDGF

プロトコール

参考文献

This product has been referenced in:
  • Feng M  et al. High glucose increases LPS-induced DC apoptosis through modulation of ERK1/2, AKT and Bax/Bcl-2. BMC Gastroenterol 14:98 (2014). WB, IHC . Read more (PubMed: 24885625) »
  • Velten M  et al. Systemic maternal inflammation and neonatal hyperoxia induces remodeling and left ventricular dysfunction in mice. PLoS One 6:e24544 (2011). WB ; Human . Read more (PubMed: 21935422) »

See all 2 Publications for this product

レビューと Q&A

Thank you for your patience in this matter.

I am very pleased to inform you that the product (positive control lysate for ab16869 - NIH3T3 cell lysate stimulated with PDGF) is now available and we are now doing the final internal necessary st...

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Thank you for your kind reminder.

I will be sending an e-mail as soon as have further information about the lysate from the Lab.

Thank you for your patience in advance.

I can now inform you that we do not have the lysate in stock currently. This is expected to be available at the earliest next month.

I am sorry for the inconvenience. If you have any further questions or concerns please do not hesitate to con...

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Thank you for your enquiry regarding ab16869.

The cells need to be stimulated and in order to be able to detect phosphorylated ERK1 + ERK2 PDGF is recommended as inducer.

Your customer may wish to take a look at this specific public...

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 cell line)
Loading amount
400000 cells
Specification
BV2 cell line
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 08 2008

Application
Western blot
Sample
Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount
20 µg
Specification
spinal cord homogenate
Treatment
sham or nerve transected
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 08 2008

Application
Western blot
Sample
Rat Cell lysate - whole cell (HAPI cell line)
Loading amount
400000 cells
Specification
HAPI cell line
Treatment
LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions
Reduced Denaturing (10 % SDA-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 11 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Microglia)
Loading amount
400000 cells
Specification
Primary Microglia
Treatment
LPS 50ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Nancy Nutile-McMenemy

Verified customer

投稿 Jun 09 2008

Thank you for filling out our technical help questionaire so thoroughly. You protocol looks correct, however I advise for this or any other antibody against a phosphorylated protein that you avoid using milk in your diluents and blocking buffer. Milk c...

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Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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