Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 [MAPK-YT] (ab50011)

製品の概要

  • 製品名
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • アプリケーション
    適用あり: ICC/IF, ELISA, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Drosophila melanogaster
    交差が予測される動物種: Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
    Sequence:

    HTGFLpTEpYVAT

  • エピトープ
    The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
  • ポジティブ・コントロール
    • Rat brain extract, Drosophila wing imaginal disc.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 44 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

画像

  • Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
    Lane 2 : no primary antibody

    Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
    Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2

    Secondary
    Goat Anti-Mouse IgG, AlkPhos Conjugate

    Predicted band size : 44 kDa
    Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
  • In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
  • ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).

参考文献

This product has been referenced in:
  • Ding Y  et al. Effects of 1,25(OH)2 D3 and vitamin D receptor on peripheral CD4(+) /CD8(+) double-positive T lymphocytes in a mouse model of systemic lupus erythematosus. J Cell Mol Med 21:975-985 (2017). WB ; Mouse . Read more (PubMed: 28063200) »
  • Liang RN  et al. Ping-Chong-Jiang-Ni Formula Induces Apoptosis and Inhibits Proliferation of Human Ectopic Endometrial Stromal Cells in Endometriosis via the Activation of JNK Signaling Pathway. Evid Based Complement Alternat Med 2017:6489427 (2017). WB ; Rat . Read more (PubMed: 28656053) »

See all 40 Publications for this product

レビューと Q&A

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Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (Tadpole limb)
Permeabilization
Yes - 1% Triton X-100
Specification
Tadpole limb
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Gufa Lin

Verified customer

投稿 Sep 12 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Aceton methanol
Username

Abcam user community

Verified customer

投稿 Feb 06 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako REALPeroxidase-Blocking Solution S2023 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated
Sample
Zebrafish Tissue sections (Liver, dysplatic cells)
Specification
Liver, dysplatic cells
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Inhye Jung

Verified customer

投稿 Jul 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neural Progenitors)
Specification
Neural Progenitors
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Goat Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 18 2012

Application
Western blot
Sample
Grasshopper Tissue lysate - other (Brain lysate)
Loading amount
10 µg
Specification
Brain lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Teresa Killick

Verified customer

投稿 Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
3 µg
Specification
Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% gradient gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Carl Atkinson

Verified customer

投稿 Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic stem cells-induced neurosphere)
Specification
Embryonic stem cells-induced neurosphere
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
10 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 22 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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