Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 [MAPK-YT] (ab50011)

製品の概要

  • 製品名Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Drosophila melanogaster
    交差が予測される動物種: Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
    Sequence:

    HTGFLpTEpYVAT

  • エピトープThe epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
  • ポジティブ・コントロール
    • Rat brain extract, Drosophila wing imaginal disc.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 44 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] 画像

  • Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
    Lane 2 : no primary antibody

    Lane 1 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2
    Lane 2 : Extract of Rat1 cells activated with 100µM Vanadate+200µM H2O2

    Secondary
    Goat Anti-Mouse IgG, AlkPhos Conjugate

    Predicted band size : 44 kDa
    Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)
  • In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
  • ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) 使用論文

This product has been referenced in:
  • Ruess DA  et al. HDACi Valproic Acid (VPA) and Suberoylanilide Hydroxamic Acid (SAHA) Delay but Fail to Protect against Warm Hepatic Ischemia-Reperfusion Injury. PLoS One 11:e0161233 (2016). WB ; Rat . Read more (PubMed: 27513861) »
  • Moeenfard M  et al. Anti-Angiogenic Properties of Cafestol and Kahweol Palmitate Diterpene Esters. J Cell Biochem 117:2748-2756 (2016). Read more (PubMed: 27129115) »

See all 24 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin)
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Aceton methanol
Username

Abcam user community

Verified customer

投稿 Feb 06 2017

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako REALPeroxidase-Blocking Solution S2023 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step Heat mediated
Sample Zebrafish Tissue sections (Liver, dysplatic cells)
Specification Liver, dysplatic cells
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Inhye Jung

Verified customer

投稿 Jul 17 2014

Thank you for your reply and further question.

This monoclonal antibody in the ascites fluid that was produced by the hybridoma within the mouse peritoneal cavity. As far as I can tell there should only be the specific antibody present in te...

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Thank you for your enquiry.

I can confirm that the total protein concentration in ab50011 should be between 15 - 55 mg/ml when measured by Biuret and that the IgG1 concentration should be between 3 - 6 mg/ml.

Regrettably we do not...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply.

I am very sorry to confirm that we sell a lot of this antibody and regrettably we now have no vials of GR121-7 left.

As previously discussed, I can confirm that our lots GR121-7 and GR121-8 are the same o...

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Thank you for taking the time to complete our questionnaire and provide the images.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have ...

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Thank you for taking the time to contact us. You enquiry has been forwarded to the scientific support team. I am sorry to hear you have had difficulty obtaining satisfactory results from this vial of antibody.

I appreciate the time you have ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"