製品の概要
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製品名Anti-ERK1 + ERK2 antibody - Loading Control
ERK1 + ERK2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ERK1 + ERK2 - Loading Control
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アプリケーション適用あり: WB, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Hamster, Zebrafish
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus ERK1 + ERK2.
(Peptide available as ab108469.)
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ポジティブ･コントロール
- This antibody gave a positive signal in human liver and kidney tissue lysates, and in the following whole cell lysates: HeLa; A431; PC12; NIH3T3; THP1; HepG2.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: PBS, BSA, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab94484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42, 44 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
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翻訳後修飾Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Entrez Gene: 399480 Zebrafish
- Omim: 176948 Human
see all -
別名
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
see all
Anti-ERK1 + ERK2 antibody - Loading Control 画像
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Western blot - Anti-ERK1 + ERK2 antibody - Loading Control (ab94484)All lanes : Anti-ERK1 + ERK2 antibody - Loading Control (ab94484) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42, 44 kDa
Observed band size : 42,44 kDa
Exposure time : 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94484 overnight at 4°C. Antibody binding was detected using a goat anti-rabbit HRP secondary antibody (ab6721), and visualised using ECL development solution ab133406
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Western blot - ERK1 + ERK2 antibody (ab94484)All lanes : Anti-ERK1 + ERK2 antibody - Loading Control (ab94484) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 6 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 7 : Human liver tissue lysate - total protein (ab29889)
Lane 8 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 9 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42, 44 kDa
Observed band size : 42,44 kDa
Additional bands at : 120 kDa,130 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 secondsSecondary antibody - goat anti-rabbit HRP (ab97080) pre-adsorbed
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Immunocytochemistry/ Immunofluorescence - ERK1 + ERK2 antibody (ab94484)ICC/IF image of ab94484 stained MCF7 cells. The cells were 4% PFA fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94484, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
Anti-ERK1 + ERK2 antibody - Loading Control (ab94484) 使用論文
ab94484 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"