製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERK1 + ERK2
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Chicken, Hamster, Xenopus laevis, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to a region of Rat ERK1 + ERK2.

  • ポジティブ・コントロール
    • Human A431 fibroblast cell lysate; Human breast cancer tissue; U251 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 41 kDa).
IP Use at an assay dependent concentration.
IHC-P 1/50.
Flow Cyt Use a concentration of 10 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメイン
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p42-MAPK antibody
    • PRKM 2 antibody
    see all

画像

  • Anti-ERK1 + ERK2 antibody (ab115799) at 1/1000 dilution + Human A431 fibroblast cell lysate

    Predicted band size : 41 kDa
  • ab115799 at 1/50 dilution staining ERK1 + ERK2 in Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.
  • Flow cytometry analysis of U251 cells using ab115799 at 10ug/ml (right) and control rabbit IgG (left).

参考文献

ab115799 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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