製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERGIC2
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 328 - 377 (TTGMLHGIGK FIVEIICCRF RLGSYKPVNS VPFEDGHTDN HLPLLENNTH) of human ERGIC2 (NP_057654).

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab83419 is purified by a peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Possible role in transport between endoplasmic reticulum and Golgi.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the ERGIC family.
  • 細胞内局在
    Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cytoplasm. Nucleus. Cycles between the endoplasmic reticulum and the Golgi. PubMed:11445006 states that it localizes to the nucleus; PubMed:17980171 shows a partial localization in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cd002 antibody
    • CD14 protein antibody
    • CDA14 antibody
    • Endoplasmic reticulum Golgi intermediate compartment protein 2 antibody
    • Endoplasmic reticulum-Golgi intermediate compartment protein 2 antibody
    • ERGI2_HUMAN antibody
    • ERGIC 2 antibody
    • ERGIC and golgi 2 antibody
    • ergic2 antibody
    • Erv41 antibody
    • MGC111152 antibody
    • PTX1 antibody
    • PTX1 protein antibody
    see all

画像

  • Anti-ERGIC2 antibody (ab83419) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83419 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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