antigen sequence: VVNELYVDDP DKDSGGKIDV SLNISLPNLH CELVGLDIQD EMGRHEVGHI DNSMKIPLNN GAGCRFEGQF SINK, corresponding to internal sequence amino acids 52-125 of Human ERGIC1
Our Abpromise guarantee covers the use of ab121574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. | |
WB | 1/250 - 1/500. |
ab121574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"