製品の概要
- 製品名Anti-ERGIC1 antibody
ERGIC1 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to ERGIC1
- アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
- 種交差性交差種: Human
- 免疫原
antigen sequence: VVNELYVDDP DKDSGGKIDV SLNISLPNLH CELVGLDIQD EMGRHEVGHI DNSMKIPLNN GAGCRFEGQF SINK, corresponding to internal sequence amino acids 52-125 of Human ERGIC1
- ポジティブ･コントロール
- Human smooth muscle tissue; RT-4, U-251 MG and Human tonsil cell lysates.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- バッファーpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab121574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. | |
| WB | 1/250 - 1/500. |
ターゲット情報
- 機能Possible role in transport between endoplasmic reticulum and Golgi.
- 配列類似性Belongs to the ERGIC family.
- 翻訳後修飾N-glycosylated.
- 細胞内局在Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi.
- Information by UniProt
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参照データベース
- Entrez Gene: 57222 Human
- SwissProt: Q969X5 Human
- Unigene: 509163 Human
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別名
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
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Anti-ERGIC1 antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121574)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121574 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human smooth muscle tissue by immunohistochemistry. -
Western blot - Anti-ERGIC1 antibody (ab121574)All lanes : Anti-ERGIC1 antibody (ab121574) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
Anti-ERGIC1 antibody (ab121574) 使用論文
ab121574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"