製品の概要

  • 製品名
    Anti-ERGIC1 antibody
    ERGIC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ERGIC1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: VVNELYVDDP DKDSGGKIDV SLNISLPNLH CELVGLDIQD EMGRHEVGHI DNSMKIPLNN GAGCRFEGQF SINK, corresponding to internal sequence amino acids 52-125 of Human ERGIC1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Human smooth muscle tissue; RT-4, U-251 MG and Human tonsil cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Possible role in transport between endoplasmic reticulum and Golgi.
  • 配列類似性
    Belongs to the ERGIC family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi.
  • Information by UniProt
  • 参照データベース
    • 別名
      • endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
      • Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
      • ER-Golgi intermediate compartment 32 kDa protein antibody
      • ERGI1_HUMAN antibody
      • ERGIC-32 antibody
      • ergic1 antibody
      • ERGIC32 antibody
      • KIAA1181 antibody
      • NET24 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121574)
      Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121574)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121574)
      ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human smooth muscle tissue by immunohistochemistry.
    • Western blot - Anti-ERGIC1 antibody (ab121574)
      Western blot - Anti-ERGIC1 antibody (ab121574)
      All lanes : Anti-ERGIC1 antibody (ab121574) at 1/250 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human plasma
      Lane 4 : Human liver lysate
      Lane 5 : Human tonsil lysate

      Developed using the ECL technique.

    参考文献

    ab121574 has not yet been referenced specifically in any publications.

    ab121574 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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