The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.
Use a concentration of 1 - 2 µg/ml.
Use a concentration of 5 - 10 µg/ml. Paraformadehyde fixed cells
関連性Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
G1 to S phase transition protein 1 homolog antibody
G1 to S phase transition protein antibody
GSPT 1 antibody
GST 1 antibody
GTP binding protein GST1 HS antibody
Transition protein 1 antibody
Anti-eRF3/GSPT1 antibody (ab49878) 使用論文
This product has been referenced in:
Geißler V et al. The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic Acids Res41:7875-88 (2013).
Read more (PubMed: 23788676) »
Peixeiro I et al. Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations. Nucleic Acids Res40:1160-73 (2012).
Read more (PubMed: 21989405) »