製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to eRF1
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow, Xenopus laevis, Arabidopsis thaliana, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eRF1.

    (Peptide available as ab31797.)

  • ポジティブ・コントロール
    • This antibody gave a positive control in the following human lysates: HeLa (Human epithelial carcinoma cell line) whole cell Jurkat (Human T cell lymphoblast-like cell line) whole cell A431 (Human epithelial carcinoma cell line) whole cell This antibody gave a positive control in the following mouse lysates: NIH 3T3 whole cell MEF1 whole cell Testis tissue Ovary tissue This antibody gave a positive control in the following rat lysate: PC12 whole cell

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab30928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-eRF1 antibody (ab30928) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-eRF1 antibody (ab30928) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49-50 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab30928 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30928, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas ab30928 staining eRF1 in human gall bladder, showing a cytoplasmic staining pattern in glandular cells. Paraffin embedded human gall bladder tissue was incubated with ab30928 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

     ab30928 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

参考文献

This product has been referenced in:
  • Zhai Y  et al. Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation. Mol Cell Biol 28:7414-26 (2008). WB ; Human . Read more (PubMed: 18936174) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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