Anti-ERF (phospho T526) 抗体 (ab59178)

製品の概要

  • 製品名
    Anti-ERF (phospho T526) antibody
    ERF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ERF (phospho T526)
  • 特異性
    Detects endogenous levels of ERF only when phosphorylated at threonine 526.
  • アプリケーション
    適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of threonine 526 (PLTPPR) of human ERF

  • ポジティブ・コントロール
    • Human lung carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

ターゲット情報

  • 機能
    Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • 組織特異性
    Highest levels in testis, ovary, pancreas, and heart.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Anti-ERF (phospho T526) antibody 画像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59178 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.

プロトコール

Anti-ERF (phospho T526) antibody (ab59178) 使用論文

ab59178 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
35 µg
Specification
HEK293 cells
Treatment
100ng/ml nocodazole 6 hours
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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