製品の概要

  • 製品名Anti-ERF antibody
    ERF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ERF
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 360-409 ((VPSSASSSSS SSSSPFKFKL QRPPLGRRQR AAGEKAVAAA DKSGGSAGGL) of human ERF (AAA86686).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • 組織特異性Highest levels in testis, ovary, pancreas, and heart.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Anti-ERF antibody 画像

  • Anti-ERF antibody (ab87039) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : ~59 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

Anti-ERF antibody (ab87039) 使用論文

ab87039 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"