Anti-ERF 抗体 (ab84773)
Key features and details
- Rabbit polyclonal to ERF
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ERF antibody
ERF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERF -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region between residue 498 and 548 of human ERF (NP_006485.2).
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ポジティブ・コントロール
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma Mouse Controls: Renal Cell Carcinoma, Teratoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab84773 was affinity purified using an epitope specific to ERF immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84773の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
特記事項 |
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Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation. -
組織特異性
Highest levels in testis, ovary, pancreas, and heart. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻訳後修飾
Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 789798 Cow
- Entrez Gene: 2077 Human
- Entrez Gene: 13875 Mouse
- Entrez Gene: 292721 Rat
- Omim: 611888 Human
- SwissProt: P50548 Human
- SwissProt: P70459 Mouse
- Unigene: 655969 Human
see all -
別名
- ERF antibody
- ERF_HUMAN antibody
- ETS domain containing transcription factor ERF antibody
see all
画像
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Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of human ovarian tumor using a 1/100 dilution of ab84773. Detection: DAB staining
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Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/500 dilution of ab84773. Detection: DAB staining
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84773 は論文での使用が確認できていません。