製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to ERF
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment with tag: LGRGSVSDCS DGTSELEEPL GEDPRARPPG PPDLGAFRGP PLARLPHDPG VFRVYPRPRG GPEPLSPFPV SPLAGPGSLL PPQLSPALPM TPTHLAYTPS PTLSPMYPSG , corresponding to amino acids 181-290 of Human ERF

  • ポジティブ・コントロール
    • Raw 264.7 whole cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • 組織特異性
    Highest levels in testis, ovary, pancreas, and heart.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

画像

  • Anti-ERF antibody (ab61108) at 1 µg/ml + Raw 264.7 whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa

プロトコール

参考文献

ab61108 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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