製品の概要

  • 製品名Anti-ERCC1 antibody [EP2143Y]
    ERCC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2143Y] to ERCC1
  • 特異性ab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERCC1 aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • SKBR-3 cell lysate
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
    • 関連疾患Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    • 配列類似性Belongs to the ERCC1/RAD10/SWI10 family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • COFS 4 antibody
      • COFS4 antibody
      • DNA excision repair protein ERCC 1 antibody
      • DNA excision repair protein ERCC-1 antibody
      • DNA excision repair protein ERCC1 antibody
      • ERCC 1 antibody
      • ERCC1 antibody
      • ERCC1_HUMAN antibody
      • Excision repair cross complementation group 1 antibody
      • Excision repair cross complementing 1 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
      • Excision repair protein antibody
      • RAD 10 antibody
      • RAD10 antibody
      • UV 20 antibody
      • UV20 antibody
      see all

    Anti-ERCC1 antibody [EP2143Y] 画像

    • Anti-ERCC1 antibody [EP2143Y] (ab76236) at 1/2000 dilution + SKBR-3 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Anti-ERCC1 antibody [EP2143Y] (ab76236) 使用論文

    ab76236 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab76236.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"