製品の概要
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製品名Anti-ERCC1 antibody [EP2143Y]
ERCC1 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP2143Y] to ERCC1
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由来種Rabbit
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特異性ab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.
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アプリケーション適用あり: WB, Flow Cytmore details
適用なし: ICC,IHC-P or IP -
種交差性交差種: Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERCC1 aa 1-100 (N terminal).
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ポジティブ･コントロール
- SKBR-3 cell lysate
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EP2143Y
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab76236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa). | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
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関連疾患Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
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配列類似性Belongs to the ERCC1/RAD10/SWI10 family.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 2067 Human
- Omim: 126380 Human
- SwissProt: P07992 Human
- Unigene: 435981 Human
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別名
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
画像
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![Western blot - Anti-ERCC1 antibody [EP2143Y] (ab76236)](http://a.abcam.com/ps/products/76/ab76236/Images/ab76236.jpg)
Western blot - Anti-ERCC1 antibody [EP2143Y] (ab76236)Anti-ERCC1 antibody [EP2143Y] (ab76236) at 1/2000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 36 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
The 100 kDa band may represent a heterodimer composed of ERCC1 and XPF/ERCC4.
参考文献
ab76236 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"