製品の概要

  • 製品名Anti-ERCC1 antibody
    ERCC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ERCC1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Hamster, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human ERCC1.

    (Peptide available as ab93790.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal mouse kidney tissue lysate and in the following whole cell lysates: HeLa; Jurkat.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab69398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • 関連疾患Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • 配列類似性Belongs to the ERCC1/RAD10/SWI10 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Anti-ERCC1 antibody 画像

  • All lanes : Anti-ERCC1 antibody (ab69398) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 36 kDa,55 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • Anti-ERCC1 antibody (ab69398) at 1 µg/ml + Kidney (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-ERCC1 antibody (ab69398) 使用論文

ab69398 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab69398.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"