Anti-ERCC1 抗体 (ab69398)
Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ERCC1 antibody
ERCC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERCC1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Hamster, Cow -
免疫原
Synthetic peptide corresponding to Human ERCC1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab93790) -
ポジティブ・コントロール
- This antibody gave a positive signal mouse kidney tissue lysate and in the following whole cell lysates: HeLa; Jurkat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69398の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
ターゲット情報
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機能
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
関連疾患
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
配列類似性
Belongs to the ERCC1/RAD10/SWI10 family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 615637 Cow
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Omim: 126380 Human
- SwissProt: Q1LZ75 Cow
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
see all -
別名
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
画像
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All lanes : Anti-ERCC1 antibody (ab69398) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 36 kDa, 55 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
Anti-ERCC1 antibody (ab69398) at 1 µg/ml + Kidney (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab69398 は論文での使用が確認できていません。