製品の概要

  • 製品名Anti-ERCC1 antibody [8F1]
    ERCC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8F1] to ERCC1
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human ERCC1.

  • ポジティブ・コントロール
    • Tonsil.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified from ascites.
  • ポリ/モノモノクローナル
  • クローン名8F1
  • ミエローマunknown
  • アイソタイプIgG2b
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 33-36 kDa.
IP 1/100 - 1/500.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100. 1/50 - 1/100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.
Flow Cyt Use 1µg for 106 cells.

ターゲット情報

  • 機能Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • 関連疾患Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • 配列類似性Belongs to the ERCC1/RAD10/SWI10 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Anti-ERCC1 antibody [8F1] 画像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue with ab2356 labelling ERCC1.

  • ICC/IF image of ab2356 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2356, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab2356 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2356, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue sections with ab2356 labelling ERCC1.

Anti-ERCC1 antibody [8F1] (ab2356) 使用論文

This product has been referenced in:
  • Millis SZ  et al. Molecular Profiling of Refractory Adrenocortical Cancers and Predictive Biomarkers to Therapy. Biomark Cancer 7:69-76 (2015). ICC/IF ; Human . Read more (PubMed: 26715866) »
  • Li C  et al. ERCC1 and the efficacy of cisplatin in patients with resected non-small cell lung cancer. Tumour Biol N/A:N/A (2014). IHC-P ; Human . Read more (PubMed: 25217323) »

See all 12 Publications for this product

Product Wall

I have received feedback from the source of the antibody ab2356 who informed me that the application "tested in WB" came from one of their customers and that they did not have information on the dilutions or blocking agents tested with this product. In...

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Thank you for contacting us.

I have contacted the lab regarding the protocol used to do IP for ab43. Unfortunately, I have found out that we have no information regarding how this antibody functions in IP and therefore we cannot guarantee th...

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Thank you for your enquiry and your interest in our products.

I would suggest carrying out a preliminary experiment with different dilutions of this primary antibody (i.e. 1:100, 1:500 and 1:1000) and then adjust the dilution accordingly to y...

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Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products.

Ho...

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Merci d'avoir pris le temps de m'expliquer la situation aujourd'hui au téléphone. Je suis très désolée que le ab2356 n'as pas non plus fonctionné.

Voici le lien pour les huit anticorps contre le ERCC1...

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Comme convenu j'ai mis en place l'envoi d'une unité de ab2356 en remplacement du ab59772. Le numéro de commande de remplacement gratuit de ce produit est ******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. <...

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Merci de nous avoir contactés.

Une perméabilisation d'environ 10 minutes avec 0.2% de Triton peut améliorer les résultats.

Merci de m'informer si cette suggestion s'avère êtreutile et nous me...

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Thank you for contacting us. We had customer feedback in 2006 that this antibody does not work in western blot so we decided to remove this application. Since then we have sold hundreds of units of this antibody and again we have received common fe...

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Thank you for your enquiry. The concentration is 100 ug/ml, and the antibody was purified from ascites. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"