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Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Rat ERC2.
(Peptide available as ab27861.)
Our Abpromise guarantee covers the use of ab27250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas
ab27250 staining ERC2 in Human cerebellum. The paraffin embedded human tissue was incubated with ab27250 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab27250 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab27250 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"