製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ERC2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Rat ERC2.

    (Peptide available as ab27861.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Rat brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ.
  • 細胞内局在
    Cytoplasm. Cell junction > synapse. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. In neurons, localized to synapses, and colocalizes with PCLO. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cast antibody
    • CAST1 antibody
    • CAZ-associated structural protein 1 antibody
    • Cmbp (gene name) antibody
    • Cytomatrix protein p110 antibody
    • ERC protein 2 antibody
    • Erc2 antibody
    • ERC2_HUMAN antibody
    • KIAA0378 antibody
    see all

画像

  • Anti-ERC2 antibody (ab27250) at 1 µg/ml + Rat Brain Whole Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 111 kDa
    Additional bands at : 50 kDa (possible non-specific secondary antibody binding).
  • Image courtesy of Human Protein Atlas

    ab27250 staining ERC2 in Human cerebellum. The paraffin embedded human tissue was incubated with ab27250 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab27250 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

参考文献

ab27250 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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