Anti-ErbB 4 (phospho Y1284) 抗体 (ab61059)

製品の概要

  • 製品名Anti-ErbB 4 (phospho Y1284) antibody
    ErbB 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ErbB 4 (phospho Y1284)
  • 特異性Detects endogenous levels of ErbB 4 only when phosphorylated at tyrosine 1284.
  • アプリケーション適用あり: ELISA, WB, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human ErbB 4 around the phosphorylation site of tyrosine 1284 (P-E-YP-L-S)

  • ポジティブ・コントロール
    • HeLa cells treated with 200nM EGF for 5 minutes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100000.
WB 1/500.
ICC/IF 1/100 - 1/500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21903113

ターゲット情報

  • 機能Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
  • 組織特異性Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
    Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
    Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
  • 細胞内局在Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4ICD antibody
    • ALS19 antibody
    • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
    • E4ICD antibody
    • EC 2.7.10.1 antibody
    • Erbb4 antibody
    • ERBB4 intracellular domain antibody
    • ERBB4_HUMAN antibody
    • HER 4 antibody
    • HER4 antibody
    • human epidermal growth factor receptor 4 antibody
    • Mer4 antibody
    • MGC138404 antibody
    • Oncogene ERBB4 antibody
    • p180erbB4 antibody
    • Proto-oncogene-like protein c-ErbB-4 antibody
    • Receptor protein tyrosine kinase erbB 4 precursor antibody
    • Receptor tyrosine protein kinase erbB 4 antibody
    • s80HER4 antibody
    • Tyrosine kinase type cell surface receptor HER4 antibody
    • Tyrosine kinase-type cell surface receptor HER4 antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
    • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
    • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
    • VERBB2 antibody
    see all

Anti-ErbB 4 (phospho Y1284) antibody 画像

  • ab61059 staining ErbB 4 (phospho Y1284) in rat heart tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with paraformaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% BSA + normal goat serum for 4 hours at 21°C. Samples were incubated with primary antibody (1/30 in 5% BSA + 0.05% Tween20 in TBS) for 12 hours at 4°C. ab150077, a goat anti-rabbit  Alexa 488 (1/200) was used as the secondary antibody.

    See Abreview

  • 1/100-1/500 ab61059 staining phosphorilated Erb B on HeLa cells treated with 200nM EGF for 5 minutes, in the absence (left) or presence (right) of the immunizing phosphopeptide.

Anti-ErbB 4 (phospho Y1284) antibody (ab61059) 使用論文

This product has been referenced in:
  • Rösler TW  et al. Biodistribution and brain permeability of the extracellular domain of neuregulin-1-ß1. Neuropharmacology 61:1413-8 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21903113) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA and Normal Goat Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Rat Cell (Heart sections)
Specification Heart sections
Permeabilization Yes - Tween20 0.05%
Fixative Paraformaldehyde
Username

Mr. Simon Pascual

Verified customer

投稿 Apr 15 2014

Thank you for your telephone call earlier today. According to Swissprot/Uniprot protein database site, the extracellular domain of the ErbB 4 is from amino acids 26 – 651. http://www.uniprot.org/uniprot/Q15303 We do have the following ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Primary lung epithelial cells and MLE12 cells)
Loading amount 30 µg
Specification Primary lung epithelial cells and MLE12 cells
Treatment 33nM neuregulin for 5min in primary lung epithelial cells or 0-30 min in MLE12
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7 cells)
Loading amount 25 µg
Specification MCF7 cells
Treatment 33 nm Neuregulin for 0 - 30 mins
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"