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Synthetic phosphopeptide derived from human ErbB 4 around the phosphorylation site of tyrosine 1284 (P-E-YP-L-S)
Our Abpromise guarantee covers the use of ab61059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21903113|
ab61059 staining ErbB 4 (phospho Y1284) in rat heart tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with paraformaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% BSA + normal goat serum for 4 hours at 21°C. Samples were incubated with primary antibody (1/30 in 5% BSA + 0.05% Tween20 in TBS) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/200) was used as the secondary antibody.
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